1Department of Immunology and Microbiology, University of Colorado School of Medicine, Aurora, CO, United States.īaxter 1, Sidney Ogolla 1 †, Rosemary Rochford 1, Debashis Ghosh 2, Pratyaydipta Rudra 3 § and Elena W.2Department of Biostatistics and Informatics, Colorado School of Public Health, Aurora, CO, United States.3Department of Statistics, Oklahoma State University, Stillwater, OK, United States.4Division of Allergy and Immunology, Department of Pediatrics, University of Colorado School of Medicine, Children's Hospital Colorado, Aurora, CO, United StatesĬytometry by Time-Of-Flight (CyTOF) uses antibodies conjugated to isotopically pure metals to identify and quantify a large number of cellular features with single-cell resolution.
Concatenate with uncompensated parameters when files sh. A barcoding approach allows for 20 unique samples to be pooled and processed together in one tube, reducing the intra-barcode technical variability. Learn best practices for using concatenated files with both compensated and uncompensated parameters. However, with only 20 samples per barcode, multiple barcode sets (batches) are required to address questions in robustly powered study designs. A batch adjustment procedure is required to reduce variability across batches and to facilitate direct comparison of runs performed across multiple barcodes run over weeks, months, or years.
FLOWJO CONCATENATE MANUAL
We describe a method using technical replicates that are included in each run to determine and apply an appropriate adjustment per batch without manual intervention.
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